ISO International Standard

Microbiology of the food chain — Horizontal method for the detection and enumeration of Enterobacteriaceae — Part 1: Detection of Enterobacteriaceae

ISO 21528-1:2017 specifies a method, with enrichment, for the detection of Enterobacteriaceae. It is applicable to

– products intended for human consumption and the feeding of animals, and

– environmental samples in the area of primary production, food production and food handling.

This method is applicable

– when the microorganisms sought are expected to need resuscitation by enrichment, and

– when the number sought is expected to be below 100 per millilitre or per gram of test sample.

A limitation on the applicability of ISO 21528-1:2017 is imposed by the susceptibility of the method to a large degree of variability (see Clause 11).

NOTE Enumeration can be carried out by calculation of the most probable number (MPN) after incubation in liquid medium. See Annex A.

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