ISO 21427-1:2006 specifies a method for assessing genotoxicity using Amphibia larvae (Xenopus laevis and Pleurodeles waltl). The method is applicable to: aqueous effluents; aqueous leachates; eluates of soils; eluates of industrial waste; eluates of sludges from sewage treatment; surface and ground water; water-soluble substances.